Histological Staining Techniques

Alcian blue for mucopolysaccharides
  • Dewax sections, rinse in alcohol, rinse in water.
  • Stain in 1% alcian blue in 3% acetic acid (pH2.5) - 5 minutes.
  • Wash in running tap water.
  • Rinse in distilled water.
  • Counterstain with nuclear fast red - 1 minute.
  • Rinse in distilled water.
  • Dehydrate, clear and mount.
    Results:
    Acid mucopolysaccharides - blue.
    Nuclei - red.