Histological Staining Techniques

Aldehyde fuchsin/alcian blue for sulphated and carboxylated mucins
Spicer & Meyer, 1960.
Use a positive control section.
  • Dewax sections, rinse in alcohol, rinse in water.
  • Rinse in 70% alcohol.
  • Stain in aldehyde fuchsin - 20 minutes.
  • Rinse well in 70% alcohol.
  • Rinse in tap water.
  • Stain in 1% alcian blue in 3% acetic acid - 1 minute.
  • Rinse in tap water.
  • Dehydrate, clear and mount.

Results: 


Sulphated mucins - purple.
Carboxylated mucins - blue.

Aldehyde fuchsin
* Pararosaniline - 1g
** Paraldehyde (Paracetaldehyde) - 2mls
Conc. HCl - 1ml
Ethanol - 60mls
Distilled water - 40mls
Dissolve the pararosaniline in the alcohol and distilled water mixed. Add the HCl and the paraldehyde. Allow to ripen for 2 - 7 days at room temperature then filter. Store at 4oC.

* The colour index number of pararosaniline is C.I. 42500. This should be a finely powdered pure form.
** Must be within date. Old stock may not work correctly.

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