University of Bristol
Veterinary Pathology
Laboratory Protocols
Cryotomy
Histological Staining Techniques
Histological fixatives
Peroxidase-anti-peroxidase Technique
Preparation of tissue section adhesives
Processing tissues for light microscopy (basics) (PDF 1.1mb)
Processing tissues for light microscopy (detailed)
Sample collection guidelines
Setting up the light microscope
Specimen preparation for electron microscopy
Histological Staining Techniques
A-F
G-J
K-O
P-T
U-Z
A - F
Alcian blue
Alcian blue/PAS
Alcian blue/safranine (Csaba)
Aldehyde fuchsin for pancreatic islet cells
Aldehyde fuchsin/alcian blue for sulphated and carboxylated mucins
Alizarin red S for calcium
Barrett\'s method for pituitary
Bleach for melanin
Bluing haematoxylin stained sections
Carbol chromotrope for eosinophils
Celestine blue
Congo red for amyloid
DMAB-rhodanine for copper
Dunn/Thompson for haemoglobin
Formalin pigment removal
Fouchet for bile pigments
G - J
Giemsa for blood cells and parasites
Goldner\'s Trichrome for Osteoid
Gomori\'s hexamine silver for basement membranes
Gomori\'s trichrome
Goodpasture\'s method for encephalitozoa
Gordon and Sweets\' method for reticulin
Gram for bacteria
Gram\'s iodine
Gram/Twort for bacteria
Grimelius for argyrophil cells
Grocott/Gomori hexamine silver for fungi
Haematoxylin and eosin (H&E)
Haematoxylin and eosin for cytology or frozen sections
Harris\' haematoxylin
High iron diamine/alcian blue for sulphated and carboxylated mucins
Holmes\' for axons and muscle striations
H.V.G. (Haematoxylin/van Gieson or Weigert/van Gieson)
K - O
Leishman for blood cells and parasites
Lugol\'s iodine
Luxol fast blue/cresyl violet
Macchiavello for rickettsieae
Masson/Fontana for melanin
Masson\'s trichrome
Mayer\'s haematoxylin
Melanin bleach
Methenamine silver (Gomori)
Methyl green/pyronin (M.G.P.) for R.N.A. & D.N.A.
Miller\'s elastin stain (E.V.G.)
Mallory\'s phosphotungstic acid haematoxylin for glial fibres
Mallory\'s phosphotungstic acid haematoxylin for non-C.N.S. tissues
M.S.B.(martius/scarlet/blue) for connective tissue and fibrin
Naumenko/Feigin (modification of Cajal) for astrocytes
Nuclear fast red counterstain
Oil red O for fats
P - T
Palmgren for peripheral nerve fibres
P.A.S. (periodic acid Schiff) for carbohydrates
Perls\' Prussian blue for ferric salts
Phloxine tartrazine for viral inclusions
Pigment identification charts
Reticulin (Gordon & Sweets)
Rubeanic acid for copper
Safranine O/fast green for cartilage
Schmorl for lipofuscins
Scott\'s tap water substitute
Sirius red for amyloid
Solochrome cyanine for myelin
Toluidine blue for mast cells
Toluidine blue for amyloid
Turnbull\'s blue reaction for ferrous salts
U - Z
Von Kossa for calcium
Van Gieson\'s stain
Warthin & Starry for Tyzzer\'s disease and spirochaetes
Weigert\'s iron haematoxylin
Wright\'s stain for blood films
Weigert/van Gieson"
Ziehl-Neelsen (Z.N.) for tubercle bacilli
Ziehl-Neelsen (Z.N.) modified for lipofuscin
Z.N./Wade-Fite variant for leprosy bacilli
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