Histological Staining Techniques

Weigert's iron haematoxylin
Weigert 1904

Solution A

  •      1% haematoxylin in 100% alcohol.
Solution B
  •      30% ferric chloride (anhydrous) - 4mls
  •      concentrated hydrochloric acid - 1ml
  •      distilled water - 95mls
Working Solution
  • Add equal quantities of solution A to solution B mix and use immediately.

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