Histological Staining Techniques

Lendrum et al, 1962.


Nuclei - brown/black.
Collagen - blue.
Red blood cells - yellow.
Muscle and fibrin - red.

Martius yellow solution
0.2g martius yellow
100mls. 95% alcohol
2g phosphotungstic acid
Dissolve the phosphotungstic acid in the alcohol before adding the martius yellow.

Crystal ponceau solution
1% crystal ponceau 6R in 2% acetic acid made up in deionised water.

Methyl blue solution
0.5% methyl blue in 1% acetic acid

Note: Deionised water is used in place of distilled water for this method to alleviate problems of non-specific and/or difficult differentiation of the red stain.

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