Histological Staining Techniques

Bluing
  • When a method includes an instruction to "wash and bue" it means to wash haematoxylin stained sections in tap water until they turn from red to blue. This is a reaction to the pH of the water - if your tap water is neutral or acid you will have to use a solution called Scott's Tap Water Substitute to blue the sections.  To prepare Scott's add 2g potassium bicarbonate and 20g magnesium sulphate to 1 litre of distilled water.

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