Laboratory Protocols

• Cryotomy
• Histological Staining Techniques
  • Index
• Histological fixatives
• Peroxidase-anti-peroxidase Technique
• Preparation of tissue section adhesives
• Processing tissues for light microscopy (basics) (PDF 1.1mb)
• Processing tissues for light microscopy (detailed)
• Sample collection guidelines
• Setting up the light microscope
• Specimen preparation for electron microscopy

Histological Staining Techniques

Lugol's iodine

• Iodine - 5g.
• Potassium iodide - 10g
• Distilled water - 500ml
• Place the iodine and potassium iodide in a small quantity of the water and allow them to dissolve fully before adding the remainder.

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