Histological Staining Techniques

Dunn/Thompson for haemoglobin
Dunn & Thompson, 1945.
Use a positive control section.
  • Dewax sections, rinse in alcohol, rinse in water.
  • Stain in a mixture of equal parts 1% alcoholic haematoxylin (ripened, not fresh) and 10% potassium alum - 15 minutes. This solution may crystallise but is still usable.
  • Rinse briefly in tap water.
  • Mordant in 5% iron alum - 1 minute.
  • Rinse briefly in tap water.
  • Restain in the solution used at the second step - 10 minutes.
  • Rinse briefly in tap water.
  • Stain in van Gieson's stain - 15 minutes.
  • Differentiate in 95% alcohol - 3 minutes.
  • Dehydrate, clear and mount.

Results: 


Red blood cells - greenish black.
Haemoglobin castes or phagocytosed haemoglobin - lighter shades of green.
Collagen - red.
Muscle and cytoplasm - yellow.

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