Laboratory Protocols

• Cryotomy
• Histological Staining Techniques
  • Index
• Histological fixatives
• Peroxidase-anti-peroxidase Technique
• Preparation of tissue section adhesives
• Processing tissues for light microscopy (basics) (PDF 1.1mb)
• Processing tissues for light microscopy (detailed)
• Sample collection guidelines
• Setting up the light microscope
• Specimen preparation for electron microscopy

Histological Staining Techniques

• Dewax sections, rinse in alcohol, rinse in water.
• Stain in 0.5% aqueous toluidine blue - 30 seconds.
• Wash in tap water.
• Dehydrate, clear and mount.

Results: 

Tissue - varying shades of blue.
Mast cell granules - red/purple.

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