Laboratory Protocols

• Cryotomy
• Histological Staining Techniques
  • Index
• Histological fixatives
• Peroxidase-anti-peroxidase Technique
• Preparation of tissue section adhesives
• Processing tissues for light microscopy (basics) (PDF 1.1mb)
• Processing tissues for light microscopy (detailed)
• Sample collection guidelines
• Setting up the light microscope
• Specimen preparation for electron microscopy

Histological Staining Techniques

Wolman, 1971, Francis, 1982.
Use a positive control section.

• Dewax sections, rinse in alcohol, rinse in water.
• Stain in 1% toluidine blue in 50% isopropanol (propan-2-ol) at 37^oC - 30 minutes.
• Blot carefully.
• Dehydrate in 100% isopropanol.
• Clear and mount.

Results:

Amyloid shows dark red birefringence under polarised light.

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