Laboratory Protocols

• Cryotomy
• Histological Staining Techniques
  • Index
• Histological fixatives
• Peroxidase-anti-peroxidase Technique
• Preparation of tissue section adhesives
• Processing tissues for light microscopy (basics) (PDF 1.1mb)
• Processing tissues for light microscopy (detailed)
• Sample collection guidelines
• Setting up the light microscope
• Specimen preparation for electron microscopy

Histological Staining Techniques

Quick H&E for cytology or frozen sections

• Fix slide in acetic alcohol (3% acetic acid in 95% methanol) - 1 minute.
• Wash in water.
• Stain in Harris' haematoxylin - 1 minute.
• Wash and blue in tap water - 1 minute.
• Counterstain in 1% eosin - 2 minutes.
• Wash in running tap water - 10 seconds.
• Dehydrate, clear and mount.

Results:

Nuclei - blue.
Other tissue components - shades of red and pink.

Return to index page