Quick H&E for cytology or frozen sections
- Fix slide in acetic alcohol (3% acetic acid in 95% methanol) - 1 minute.
- Wash in water.
- Stain in Harris' haematoxylin - 1 minute.
- Wash and blue in tap water - 1 minute.
- Counterstain in 1% eosin - 2 minutes.
- Wash in running tap water - 10 seconds.
- Dehydrate, clear and mount.