Histological Staining Techniques

Quick H&E for cytology or frozen sections
  • Fix slide in acetic alcohol (3% acetic acid in 95% methanol) - 1 minute.
  • Wash in water.
  • Stain in Harris' haematoxylin - 1 minute.
  • Wash and blue in tap water - 1 minute.
  • Counterstain in 1% eosin - 2 minutes.
  • Wash in running tap water - 10 seconds.
  • Dehydrate, clear and mount.

Results:


Nuclei - blue.
Other tissue components - shades of red and pink.

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