Laboratory Protocols

• Cryotomy
• Histological Staining Techniques
  • Index
• Histological fixatives
• Peroxidase-anti-peroxidase Technique
• Preparation of tissue section adhesives
• Processing tissues for light microscopy (basics) (PDF 1.1mb)
• Processing tissues for light microscopy (detailed)
• Sample collection guidelines
• Setting up the light microscope
• Specimen preparation for electron microscopy

Histological Staining Techniques

Harris' haematoxylin
Harris 1900.

• Haematoxylin - 5g.
• 100% alcohol - 50mls
• Potassium alum - 100g.
• Distilled water - 1 litre
• Mercuric oxide - 2.5g.
• Glacial acetic acid - 40mls

Dissolve the potassium alum in the water by warming and stirring.
Dissolve the haematoxylin in the alcohol and add.
Bring rapidly to the boil remove from the heat and add the mercuric oxide.
Cool, add the acetic acid and filter.
Ready for use immediately.

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