Macchiavello for rickettsieae
Use a positive control.
- Take to water
- Stain in freshly prepared 0.25% *basic fuchsin in pH7.2 phosphate buffer - 30 minutes
- Differentiate in 0.5% citric acid - 30 seconds to 1 minute or more (check microscopically)
- Counterstain in 1% aqueous methylene blue - 15 to 30 seconds
- Wash in water
- Dehydrate, clear and mount.
Rickettsieae - red
Certain viral inclusions - red
Background - blue
*Synonyms basic fuchsin / basic red 9 / pararosaniline = C.I. 42500