Histological Staining Techniques

Permanganate bleach to remove melanin
(Use poly-l-lysine coated slides.)
  • Dewax sections, rinse in alcohol, rinse in water.
  • Bleach in 0.25% potassium permanganate - 20 minutes.
  • Rinse in distilled water.
  • Decolourise in 1% oxalic acid - 1 minute.
  • Rinse in distilled water.
  • Either:
  • Rinse in  distilled water.
  • Dehydrate, clear & mount.

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