Histological Staining Techniques

Miller's elastin stain (E.V.G.)

Miller, 1971.


Elastin and mast cell granules - black/purple.
Collagen - red.
Muscle and cytoplasm - yellow.

Miller's stain

Victoria blue - 2g
New fuchsin - 2g
Crystal violet - 2g
Dissolve the dyes in 400mls of distilled water.
Add in the following order:
Resorcin - 8g
Dextrin - 2g
Freshly prepared 30% aqueous ferric nitrate - 100mls

Boil for 5 minutes and filter whilst still hot.
Transfer the precipitate plus the filter paper to the original flask and redissolve in 400mls of 95% alcohol.
Boil (using a hotplate NOT a naked flame) for 15-20 minutes.
Cool, filter and make up to 400mls with 95% alcohol.
Add 4mls of concentrated HCl.
This solution keeps very well and if stored in a sealed coplin jar can be reused many times.

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