Histological Staining Techniques

Puchtler, Sweat, Levine, 1962.
Cut sections at 6µm. Do not use gelatine coated slides. Use a positive control section.

Results: 


Amyloid, eosinophils, elastin - brick red. Amyloid also displays apple-green birefringence under polarized light.
Nuclei - blue.
Background - clear/pale pink.

Stock solution A
Saturated sodium chloride in 80% alcohol
Working solution = 50ml stock solution A + 0.5ml 1% aqueous sodium hydroxide

Stock Solution B
Saturated congo red in 80% alcohol saturated with sodium chloride
Working solution = 50ml stock solution B + 0.5ml 1% aqueous sodium hydroxide

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