Histological Staining Techniques

High iron diamine/alcian blue for sulphated and carboxylated mucins (HID/AB)

Spicer, 1965.

Use a positive control section.


Sulphated mucins - brown/black.
Carboxylated mucins - blue.

HID solution

N,N-dimethyl-meta-phenylenediamine-dihydrochloride - 120mg
N,N-dimethyl-para-phenylenediamine-dihydrochloride - 20mg
Distilled water - 50mls
Freshly prepared 60% ferric chloride solution - 1.4mls

Dissolve the two diamine salts in the distilled water and then add the ferric chloride solution. Make the solution up directly into a Coplin jar for staining.

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