Goodpasture's carbol fuchsin stain for encephalitozoa Goodpasture-Perrin 1943.
Use a positive control section. Fix in Zenker or Orth, or formalin fix and post chrome with 2.5% potassium
dichromate for 2 days prior to processing.
Dewax sections, rinse in alcohol, rinse in water.
If Zenker fixed, remove mercury pigment.
Stain on a rack with carbol ailine fuchsin, heat to steaming with a
flame twice over 5 minutes.
Rinse quickly with tap water.
Decolourise with 38% analar formaldehyde in a sealed coplin jar until no more
colour is removed - 45 minutes.
Rinse with tap water.
Counterstain with saturated aqueous picric acid for 1 minute.