Histological Staining Techniques

Ziehl, Neelsen, 1882, 1883.
Do not celloidinise slides. Use a positive control section. Gelatinised slides may be used.

Results:

Acid fast bacilli - deep magenta red.
Nuclei - dark blue.
Background - yellow.
Red blood cells - pale pink.

Note: The more usual counterstain is 0.25% methylene blue in 1% acetic acid, for 1 minute, applied after the differentiation and washing steps. This gives a pale blue background:
Acid fast bacilli - deep magenta red.
Nuclei - blue
Background - pale blue to unstained.
Red blood cells - pale pink.

Carbol fuchsin
Dissolve 1g of *basic fuchsin in 10mls of 100% alcohol and add 100mls of 5% aqueous phenol. Mix well for several hours and filter before use.

*Synonyms basic fuchsin / basic red 9 / pararosaniline = C.I. 42500

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