Histological Staining Techniques

Luxol fast blue/cresyl violet

Kluver and Barrera, 1954.

Use 15µm sections.

(higher magnification)

Myelin - blue.
Red blood cells - turquoise.
Nuclei and nissl substance - purple.

* For the 2 hour timing use 0.05% lithium carbonate to differentiate instead of 0.005%, but be careful, it works much more quickly!

Luxol fast blue solution

Luxol fast blue MBS - 1g
95% alcohol - 1 litre
10% acetic acid - 5mls

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